Building of hFⅨ transgenic mice by spermatogenic cells

Human FⅨ expression vector pCMVⅨ was packaged by effectene^TM reagent and injected into mice seminiferous tubules with glass pipettes.The expressional frame of pCMVⅨ was examined by PCR and Southern blotting among 41 progenies.There were 2(4%) mice being integrated with hFⅨ gene into chromosomes.4.6ng/mL of hFⅨ protein was expressed in plasma of one mouse,which was tested by ELISA.We demonstrated that building of transgenic animals by spermatogonial stem cells is an efficient method.Meanwhile,it has also been proved to be an alternative choice for mammary gland bioreactor.     

Evolution of the South China Sea and monsoon history revealed in deep-sea records

As the third summary report of ODP Leg 184 to the South China Sea (SCS), this paper discusses the evolution of the East Asian monsoon and the SCS basin. A multi-proxy approach, involving geochemistry, micropale-ontology, pollen and other analyses, was adopted for reconstructing the evolutionary history of the East Asian monsoon, which was characterized by a series of paleo-climate events especially at 8, 3.2, 2.2 and 0.4 Ma. The new record indicates similar stages in the development of the East and South Asian monsoons, with an enhanced winter monsoon over East Asia being the major difference. The rich spectrums of monsoon variability from the southern SCS also reveal other characteristic features of the low latitude ocean. Evidence for the evolution of the SCS includes the hemipelagic Oligocene sediments, implying the existence of deep water environments during the early seafloor spreading stage of the SCS basin. The four major unconformities and some remarkabl ediagenetic features in upper Oligocene deposits indicate the strongest tectonic events in the region. From a careful comparison of lithologies and sedimentation rates, we conclude that the prominent differences in sedimentary environments between the southern and northern SCS were established only by ～3 Ma.     

磷钼杂多酸催化合成乳酸正丁酯

本探讨了用磷钼酸作催化剂，以乳酸和正丁醇为原料合成乳酸正丁醋，通过对各种酸醇比，催化剂用量，不同反应时间的正交试验，确定了最佳反应条件。     

C语言多维数组与指针的探讨

在C语言的学习中，数组与指针的应用是重点和难点，尤其多维数组和指针应用已成为学习和掌握C语言的一大障碍。利用消维法和图示法，通过二维、三维数组与指针的关系，延伸到多维数组的探讨，让学习C语言的人轻松的理解和接受利用指针法对多维数组元素取值的方式。     

Inhibitory effect of the adenovirus type 5 E1A protein expressed in the yeast system of the human tumor cell growth

Adenovirus 5 type E1A as a tumor suppressor gene can inhibit tumor growth and enhance the censitivity of chemotherapy and radiotherapy.E1A have the ability to integrate into the host genome,resulting in long-time expres-sion that induces Rb gene inactivation and animal cells im-mortalization.This prompted us to select the E1A protein for treatment of cancer in order to overcome the limitations of E1A gene therapy.Thus,we firstly comstructed E1A eu-caryotic expression vector (pPIC9/E1A),transformated the pichia pastoris yeast cells(GS115) and screened the high-expressing recombinant strains.The positive yeast strains were cultured in the shake flask,and induced for 3d.The crude E1A protein was purified using two steps of col-umu chromatography on HiTrap Q and HiTrap SP.The pu-rified E1A protein was identified by SDS-PAGE and Western blot.E1A protein was mostly located at cellular unclear when Cheriot delivered E1A protein into cells.The analysis in vitro indicated that the E1A protein arrested LN686 cell cycle at G2/M phase,and significantly inhibited the growth of LN686 tumor cells.The current studies firstly provided an experimental basis to further develop E1A protein for tumor treatment.     

Expression of human clotting factor Ⅸ mediated by recom- binant lentiviral vector in cultured cells and hemophilia B mice

To explore the expression of human clotting factor Ⅸ (hFⅨ) cDNA in vitro and the feasibility of gene therapy for hemophilia B mice mediated by recombinant lentiviral vector, a recombinant hFⅨ lentiviral vector driven by ubiquitin-C promoter, FUXW, and by ABP liver specific promoter, FAXW, was constructed respectively. Recombinant lentivirus was harvested from 293T cells by calcium phosphate-mediated transient cotransfection of three plasmids (transgene vector, CMV腞8.2, VSV-G). hFⅨ expression was detected in supernatant of 293T, BHK and L-02 cells infected with FUXW virus, whereas higher expression of hFⅨ levels (630 ng/106 cells/48 h) was detected only in L-02 cells infected with FAXW virus. Serum hFⅨ antigen was detected in all hemophilia B mice treated with FAXW virus by tail vein injection, an efficiency level of hFⅨ was observed (45 ng/mL, approximately 1% of normal human levels), the expression lasted for more than 60 d. The results indicated that HIV-based lentiviral vectors offer a promising approach to the gene therapy of hemophilia B.     

惠民凹陷临南地区油气输导系统及油气运移特征

油气输导系统和油气运移的特征决定油气藏形成与分布 .根据惠民凹陷临南地区的实际地质条件的分析 ,指出在异常低压的背景下 ,该研究区输导系统和油气运移聚集呈现如下特征 :( 1 )该区输导系统主要由砂体和断层组成 ,断层 (以夏口断层为主 )在油气运移中起着更重要的作用 ,且受断层面形态控制 ,两个北凸面起着汇聚油流的作用 ;( 2 )由于异常低压使得运移动力非常弱 ,油气运移表现为低速流近距离运移的特征 ,油气藏主要分布在夏口断层两侧 ,斜坡南侧高部位无油气藏     

模糊分析在油气储量产能预测中的应用--以靖安油田盘古梁区开发数据为例的分析

针对常规因素分析法在实际应用中所存在的一些问题 ,在阐述模糊分析理论特点和数学模型的同时 ,具体研究了模糊分析设计在油气储量产能预测中的应用 .通过多因素关联度分析导出影响产能开发的因素 ,从主到次依次为 :渗透率、油层厚度、加砂强度、加砂量、孔隙度等     

一种基于WEB的油藏信息系统的设计与实现

在介绍了 Timas油田油藏信息系统的整体设计目标和技术方案的基础上 ,阐述了 Timas现代油田油藏信息系统具体的设计方法和实现技术方案 ,详细介绍了 Timas油田油藏信息系统的各功能模块以及如何通过 Java实现长报表的打印技术 .并重点阐述了如何通过 JDBC实现对异构数据库的访问以及重点阐述了如何实现基于 Oracle的分布式数据库的访问